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Crabyon Safety Data Sheet

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Acute Toxicity Test (Chitin)
LD 50(lethal dose 50): Over 3,000mg/Kg

The term lethal dose 50 is defined as the dose of a substance which is fatal to 50% of the test animals. More specifically. Lethal dose 50 means that if chitin is orally administered to mice on the basis of 3,000mg/Kg (mouse weight), 50% of them die.

Mutagenicity Test (Chitin)
Mutagenicity Test: Negative
This test is conducted to determine whether or not the test animals undergo an abrupt change in the genotype of organ.
Positive: Mutagenic
Negative: not Mutagenic

Skin irritation Patch Test *
Patch Test: 1B (almost negative)

To be in compliance with JAFET (Japanese Association for the Functional Evaluation of Textile), the fabric in question has to be at least almost negative.

* A test in which material is applied and left in contact with skin surfaces for 48 hours to check tissue sensitivity.


Clinical tests of whether "CRABYON underwear" will improve patients of atopic dermatitis.

The objective is to determine whether CRABYON, with many characteristics can alleviate the symptom of atopic dermatitis.

Tester of CRABYON underwear
A doctor of medicine who runs a pediatrics in Osaka, Japan.

Test Specimen
Half-sleeved underwear of blended fabric (30% Crabyon / 70% cotton).

Testers (patients)
The doctor selected 11 patients from among his outpatients, whose symptoms are relatively stable and whom he can be reach easily for consultation.

a. Test period: January to March, 1996
b. The number of testers: 11 patients
c. Age: Children of 2 year 3 month to 11 years 3 month old.

Test Method
All 11 testers wore CRABYON underwear after obtaining their parents consent. Three pieces of underwear were given to each testers who wore them one after another and were washed after each use. The doctor and parents saw each tester every two weeks. After eight weeks, he evaluated whether or not the CRABYON underwear improved patients of atopic dermatitis.


Efficacy Evaluation

Improved considerably
Improved a little
Remain unchanged
a. Doctor's Comments
He recorded the skin conditions such as itch, dryness and scratches every two weeks and classified them into five categories:
Very effective
Somewhat effective
Not effective
b. Overall Evaluation
Considering the skin conditions, use of medicine and food, he classified the underwear's effectiveness into five categories:

Test Results

2-3 years old
3 persons
11 persons
a. Testers Age Group
5 (improved considerably)
"0" persons
4 (improved)
3 (somewhat improved)
2 (remain unchanged)
1 (aggravated)
b. Test results
He classified the testers skin conditions as follows:

He classified the effectiveness of CRABYON underwear as follows:
5 (very effective)
1 person (s)
4 (effective)
3 (somewhat effective)
2 (not effective)
1 (unknown)
c. Side effects
All 11 testers experienced no side effects of CRABYON at the end of two month-long wear test. At the end of the test, it is considered that the CRABYON underwear is safe.

d. Time dependent change of skin conditions
Table 1 and Fig. 9 show how skin conditions changed over time. (Weighted average of five categories)


Table1 - Time dependent change of skin conditions
1 day
4 week
8 week

Crabyon influences skin conditions positively - change diagram


a. The CRABYON underwear, which 11 testers wore during the clinical test, is made of 30% CRABYON / 70% cotton fabric. Treatment and sewing were done by the process now commercially available on the market.

The test period of January to March, 1996, was dry and cold when people were susceptible to cold, so we must admit that the time was rather unfavorable for us to determine whether the underwear served to alleviate the symptom of atopic dermatitis. All 11 testers were selected from among those who have relatively stable, slight to less symptoms to atopic dermatitis and who can see the doctor regularly.

b. Among the 11 testers, there was one tester whose symptom subsided considerably, one tester whose skin conditions improved, four testers whose skin conditions improved and two testers whose symptoms of atopic dermatitis could not be evaluated accurately because 

(1) one of them developed the symptom of asthma, being in bad physical condition, 
(2) one of them changed medicines, making it difficult to determine the effectiveness of the CRABYON underwear and 
(3) a mother who was too conscious of her child's atopic dermatitis overdosed her child, hoping it might improve the skin condition.

Even after considering the existence of such a mother, more than half of the 11 testers somewhat improved in skin conditions and one tester improved considerably; we think that the underwear is effective in improving the patients of atopic dermatitis.

c. All the CRABYON underwear used in clinical tests was dyed with fluorescent dyes and made with conventional sewing process. Some of the 11 testers parents said that, since the CRABYON underwear was soft to the skin even after repeated washings, they were able to use it without any worry.


Test of plant disease-resistance

Test method
Moistened cucumber seeds were planted on rock wool bed, bark block bed, CRABYON-non-woven fabric bed in a glass-house. After two weeks plant disease bacteria, Phytophorame Langeae, was inoculated into cucumbers and resistance was checked one week after inoculation.

As is shown in the photograph, CRABYON bed proved to be more resistant to the disease than other beds.

The biodegradability of CRABYON

Since CRABYON is made of organic material Chitin/Chitosan and cellulose, its biodegradability is excellent. It begins to be decomposed by bacteria 2 weeks to 2 months after being buried underground, which is almost the same as in the case of natural fallen leaves.

Result of test. Rock wool: left. Bark block: center. CRABYON: right

Fig.10 Result of test. Rock wool: left. Bark block: center. CRABYON: right.
Test of biodegradability

Fig. 11 Test of biodegradability


Main Chitosan page can be found here


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